Povidone-iodine as a sporicide.

Thioglycollate Broth Medium was used for culturing the test bacteria. Thioglycollate Broth Medium containing 0.5% sodium thiosulfate, O.S^o Tween ® 80, and 0.07% lecithin was used as the transplant medium. The sodium thiosulfate inactivates free iodine which mav be carried over. The Tween ® 80 and lecithin serve as inactivators for the quaternary compounds and hexachlorophene, and thioglycollate as inactivator for the mercurials.